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Preparation of a Brucella multiepitope fusion protein based on bioinformatics and its application in serological diagnosis of human brucellosis

  • Suárez-Esquivel, M., Chaves-Olarte, E. & Moreno, E. Guzmán-Verri, C. Brucella genomics: macro and micro evolution. Int. J. Mol. Sci. 21, 7749 (2020).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ali, S. et al. Molecular epidemiology of brucellosis in Asia: Insights from genotyping analyses. Vet. Res. Commun. 48, 3533–3550 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Nabi, I. et al. Serological, phenotypic and molecular characterization of brucellosis in small ruminants in Northern Algeria. Front. Microbiol. 15, 1505294 (2024).

    Article 
    PubMed 

    Google Scholar
     

  • Franco, M. P., Mulder, M., Gilman, R. H. & Smits, H. L. Human brucellosis. Lancet Infect. Dis. 7, 775–786 (2007).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Pinn-Woodcock, T. et al. A one-health review on brucellosis in the united States. J. Am. Vet. Med. Assoc. 261, 451–462 (2023).

    Article 
    PubMed 

    Google Scholar
     

  • Almuzaini, A. M. et al. Seroprevalence of brucellosis in camels and humans in the Al-qassim region of Saudi Arabia and its implications for public health. AMB Express. 15, 22 (2025).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Emmanouil, M. et al. Epidemiological investigation of animal brucellosis in domestic ruminants in Greece from 2015 to 2022 and genetic characterization of prevalent strains. Pathog (Basel Switz). 13, 720 (2024).


    Google Scholar
     

  • Qureshi, K. A. et al. Brucellosis: Epidemiology, pathogenesis, diagnosis and treatment-a comprehensive review. Ann. Med. 55, 2295398 (2023).

    Article 
    PubMed 

    Google Scholar
     

  • Freire, M. L., de Assis, M., Silva, T. S., Cota, G. & S. N. & Diagnosis of human brucellosis: Systematic review and meta-analysis. PLoS Negl. Trop. Dis. 18, e0012030 (2024).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Yagupsky, P., Morata, P. & Colmenero, J. D. Laboratory diagnosis of human brucellosis. Clin. Microbiol. Rev. 33, e00073–e00019 (2019).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Andrade, R. S. et al. Accuracy of serological tests for bovine brucellosis: A systematic review and meta-analysis. Prev. Vet. Med. 222, 106079 (2024).

    Article 
    PubMed 

    Google Scholar
     

  • Ml, F., Ts, M. A., Sn, S. & G, C. Diagnosis of human brucellosis: systematic review and meta-analysis. PLoS Negl. Trop. Dis 18, (2024).

  • Rahbarnia, L., Farajnia, S., Naghili, B. & Saeedi, N. Comparative evaluation of nested polymerase chain reaction for rapid diagnosis of human brucellosis. Arch. Razi Inst. 76, 203–211 (2021).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Becker, G. N. & Tuon, F. F. Comparative study of IS711 and bcsp31-based polymerase chain reaction (PCR) for the diagnosis of human brucellosis in whole blood and serum samples. J. Microbiol. Methods. 183, 106182 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Zeybek, H., Acikgoz, Z. C., Dal, T. & Durmaz, R. Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis. Folia Microbiol. 65, 353–361 (2020).

    Article 
    CAS 

    Google Scholar
     

  • Novak, A. et al. Development of a novel glycoprotein-based immunochromatographic test for the rapid serodiagnosis of bovine brucellosis. J. Appl. Microbiol. 132, 4277–4288 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Li, G. et al. Rapid detection of brucellosis using a quantum dot-based immunochromatographic test strip. PLoS Negl. Trop. Dis. 14, e0008557 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gusi, A. M. et al. Comparative performance of lateral flow immunochromatography, iELISA and Rose Bengal tests for the diagnosis of cattle, sheep, goat and swine brucellosis. PLoS Negl. Trop. Dis. 13, e0007509 (2019).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Loubet, P. et al. Diagnosis of brucellosis: Combining tests to improve performance. PLoS Negl. Trop. Dis. 18, e0012442 (2024).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lyimo, B. et al. Seroprevalence and risk factors for brucellosis amongst livestock and humans in a multi-herd ranch system in Kagera, tanzania. Front. Public. Health. 12, 1478494 (2024).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bonfini, B. et al. Cross-reactivity in serological tests for brucellosis: a comparison of immune response of escherichia coli O157:H7 and yersinia enterocolitica O:9 vs brucella spp. Vet. Ital. 54, 107–114 (2018).

    PubMed 

    Google Scholar
     

  • O’Grady, D., Kenny, K., Power, S., Egan, J. & Ryan, F. Detection of yersinia enterocolitica serotype O:9 In the faeces of cattle with false positive reactions in serological tests for brucellosis in Ireland. Vet. J. (Lond Engl. : 1997). 216, 133–135 (2016).

    Article 

    Google Scholar
     

  • Lu, J. et al. Novel vertical flow immunoassay with Au@PtNPs for rapid, ultrasensitive, and on-site diagnosis of human brucellosis. ACS Omega. 8, 29534–29542 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wu, Q. et al. Study on antigenic protein Omp2b in combination with Omp31 and BP26 for serological detection of human brucellosis. J. Microbiol. Methods. 205, 106663 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Zhang, H. et al. Expression and regulation of the ery Operon of brucella melitensis in human trophoblast cells. Exp. Ther. Med. 12, 2723–2728 (2016).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Barbier, T. et al. Erythritol feeds the Pentose phosphate pathway via three new isomerases leading to D-erythrose-4-phosphate in brucella. Proc. Natl. Acad. Sci. U S A. 111, 17815–17820 (2014).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Jf, S. & Dc, R. Inhibition of growth by erythritol catabolism in brucella abortus. J Bacteriol 124, (1975).

  • Hop, H. T. et al. Immunization of BALB/c mice with a combination of four Recombinant brucella abortus proteins, AspC, Dps, InpB and Ndk, confers a marked protection against a virulent strain of brucella abortus. Vaccine 36, 3027–3033 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Arayan, L. T. et al. Substantial protective immunity conferred by a combination of brucella abortus Recombinant proteins against brucella abortus 544 infection in BALB/c mice. J. Microbiol. Biotechnol. 29, 330–338 (2019).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Yu, H., Rao, X. & Zhang, K. Nucleoside diphosphate kinase (ndk): A pleiotropic effector manipulating bacterial virulence and adaptive responses. Microbiol. Res. 205, 125–134 (2017).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Rocha, R. O. & Wilson, R. A. Magnaporthe oryzae nucleoside diphosphate kinase is required for metabolic homeostasis and redox-mediated host innate immunity suppression. Mol. Microbiol. 114, 789–807 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Vande Voorde, J. et al. Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target. Nat. Metab. 5, 1303–1318 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Saidu, A. S. et al. Studies on intra-ocular vaccination of adult cattle with reduced dose brucella abortus strain-19 vaccine. Heliyon 8, e08937 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • de Carvalho, G. et al. Detection of brucella S19 vaccine strain DNA in domestic and wild ungulates from Brazilian Pantanal. Curr. Microbiol. 81, 333 (2024).

    Article 

    Google Scholar
     

  • Jl, S., Aa, H., Lr, S. B. & Rs, K. S. Diagnostic utility of LAMP PCR targeting bcsp-31 gene for human brucellosis infection. Indian J. Med. Microbiol 44, (2023).

  • Palacios-Chaves, L. et al. Brucella abortus ornithine lipids are dispensable outer membrane components devoid of a marked pathogen-associated molecular pattern. PLOS One. 6, e16030 (2011).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Chain, P. S. G. et al. Whole-genome analyses of speciation events in pathogenic brucellae. Infect. Immun. 73, 8353–8361 (2005).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Malik, A. A., Ojha, S. C., Schaduangrat, N. & Nantasenamat, C. ABCpred: A webserver for the discovery of acetyl- and butyryl-cholinesterase inhibitors. Mol. Divers. 26, 467–487 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • B, Y. & C, Z. D, Z., S, L. SVMTriP: a method to predict B-cell linear antigenic epitopes. Methods Mol. Biol. (Clifton N J) 2131, (2020).

  • Zheng, D., Liang, S. & Zhang, C. B-cell epitope predictions using computational methods. Methods Mol. Biol. (Clifton N J). 2552, 239–254 (2023).

    Article 

    Google Scholar
     

  • Fernandes, J. D. et al. The UCSC SARS-CoV-2 genome browser. Nat. Genet. 52, 991–998 (2020).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zhang, J. et al. Bioinformatics analysis of the antigenic epitopes of L7/L12 protein in the B- and T-cells active against brucella melitensis. Access. Microbiol. 6, 786v3 (2024).

    Article 

    Google Scholar
     

  • Bernhardt, G. V., Bernhardt, K., Shivappa, P. & Pinto, J. R. T. Immunoinformatic prediction to identify staphylococcus aureus peptides that bind to CD8 + T-cells as potential vaccine candidates. Vet. World. 17, 1413–1422 (2024).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Pillay, K., Chiliza, T. E., Senzani, S., Pillay, B. & Pillay, M. Silico design of mycobacterium tuberculosis multi-epitope adhesin protein vaccines. Heliyon 10, e37536 (2024).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wu, Q. et al. Preparation and application of a brucella multiepitope fusion protein based on bioinformatics and tandem mass tag-based proteomics technology. Front. Immunol. 15, 1509534 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Foroutan, M. & Shadravan, M. M. Completing the pieces of a puzzle: in-depth probing of Toxoplasma gondii Rhoptry protein 4 as a promising target for vaccination using an in-silico approach. Clin. Exp. Vaccine Res. 13, 359–369 (2024).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Han, J. et al. Ligand and G-protein selectivity in the κ-opioid receptor. Nature 617, 417–425 (2023).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kim, H. et al. Irisin mediates effects on bone and fat via ΑV integrin receptors. Cell 178, 507–508 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Conde, D., Garrido, P. F., Calvelo, M., Piñeiro, Á. & Garcia-Fandino, R. Molecular dynamics simulations of transmembrane Cyclic peptide nanotubes using classical force fields, hydrogen mass repartitioning, and hydrogen isotope exchange methods: A critical comparison. Int. J. Mol. Sci. 23, 3158 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

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