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Performance and suitability of wastewater based-surveillance for SARS-CoV-2 RNA in public schools

  • Parkins, M. D. et al. Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond. Clin. Microbiol. Rev. 37, e00103–00122. https://doi.org/10.1128/cmr.00103-22 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Spurbeck, R. R., Minard-Smith, A. & Catlin, L. Feasibility of neighborhood and building scale wastewater-based genomic epidemiology for pathogen surveillance. Sci. Total Environ. 789, 147829. https://doi.org/10.1016/j.scitotenv.2021.147829 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zahedi, A., Monis, P., Deere, D. & Ryan, U. Wastewater-based epidemiology-surveillance and early detection of waterborne pathogens with a focus on SARS-CoV-2, cryptosporidium and giardia. Parasitol. Res. 120, 4167–4188. https://doi.org/10.1007/s00436-020-07023-5 (2021).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Galani, A. et al. SARS-CoV-2 wastewater surveillance data can predict hospitalizations and ICU admissions. Sci. Total Environ. 804, 150151. https://doi.org/10.1016/j.scitotenv.2021.150151 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Schmitz, B. W. et al. Enumerating asymptomatic COVID-19 cases and estimating SARS-CoV-2 fecal shedding rates via wastewater-based epidemiology. Sci. Total Environ. 801, 149794. https://doi.org/10.1016/j.scitotenv.2021.149794 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Goverment-of-Canada. Wastewater Monitoring Dashboard, (2025). https://health-infobase.canada.ca/wastewater/about.html

  • CDC. National Wastewater Surveillance System (NWSS), (2025). https://www.cdc.gov/nwss/about.html

  • Naughton, C. C. et al. Show us the data: global COVID-19 wastewater monitoring efforts, equity, and gaps. FEMS Microbes. 4 https://doi.org/10.1093/femsmc/xtad003 (2023).

  • Mahmoudi, T., Naghdi, T., Morales-Narváez, E. & Golmohammadi, H. Toward smart diagnosis of pandemic infectious diseases using wastewater-based epidemiology. Trends Analyt Chem. 153, 116635. https://doi.org/10.1016/j.trac.2022.116635 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Acosta, N. et al. Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population. Water Res. 220, 118611. https://doi.org/10.1016/j.watres.2022.118611 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rubio-Acero, R. et al. Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich—one year of experience. Sci. Total Environ. 797, 149031. https://doi.org/10.1016/j.scitotenv.2021.149031 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Barrios, R. E., Lim, C., Kelley, M. S. & Li, X. SARS-CoV-2 concentrations in a wastewater collection system indicated potential COVID-19 hotspots at the zip code level. Sci. Total Environ. 800, 149480. https://doi.org/10.1016/j.scitotenv.2021.149480 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rios, G. et al. Monitoring SARS-CoV-2 variants alterations in nice neighborhoods by wastewater nanopore sequencing. Lancet Reg. Health Eur. 100202–100202. https://doi.org/10.1016/j.lanepe.2021.100202 (2021).

  • Carrillo-Vega, M. F., Salinas-Escudero, G., Garcia-Pena, C., Gutierrez-Robledo, L. M. & Parra-Rodriguez, L. Early estimation of the risk factors for hospitalization and mortality by COVID-19 in Mexico. PLoS One. 15, e0238905. https://doi.org/10.1371/journal.pone.0238905 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Castro-Gutierrez, V. et al. Monitoring occurrence of SARS-CoV-2 in school populations: a wastewater-based approach. PLOS One. 17, e0270168. https://doi.org/10.1371/journal.pone.0270168 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Acosta, N. et al. A multicenter study investigating SARS-CoV-2 in tertiary-care hospital wastewater. Viral burden correlates with increasing hospitalized cases as well as hospital-associated transmissions and outbreaks. Water Res. 201, 117369. https://doi.org/10.1016/j.watres.2021.117369 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Acosta, N. et al. Surveillance for SARS-CoV-2 and its variants in wastewater of tertiary care hospitals correlates with increasing case burden and outbreaks. J. Med. Virol. 95, e28442. https://doi.org/10.1002/jmv.28442 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lee, B. E. et al. Early warning and rapid public health response to prevent COVID-19 outbreaks in long-term care facilities (LTCF) by monitoring SARS-CoV-2 RNA in LTCF site-specific sewage samples and assessment of antibodies response in this population: prospective study protocol. BMJ Open. 11, e052282. https://doi.org/10.1136/bmjopen-2021-052282 (2021).

    Article 
    PubMed 

    Google Scholar
     

  • Pang, X. et al. Early warning COVID-19 outbreak in long-term care facilities using wastewater surveillance: correlation, prediction, and interaction with clinical and serological statuses. Lancet Microbe. 5 https://doi.org/10.1016/S2666-5247(24)00126-5 (2024).

  • ECDC. COVID-19 in Children and the Role of School Settings in Transmission—Second Update, (2021). https://www.ecdc.europa.eu/en/publications-data/children-and-school-settings-covid-19-transmission

  • Lewis, D. Why schools probably aren’t COVID hotspots. Nature 587, 17. https://doi.org/10.1038/d41586-020-02973-3 (2020).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Gray, D. J., Williams, G. M. & McManus, D. P. COVID-19, children and schools: overlooked and at risk. Med. J. Aust. 214, 188. https://doi.org/10.5694/mja2.50937 (2021).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Cui, X. et al. A systematic review and meta-analysis of children with coronavirus disease 2019 (COVID-19). J. Med. Virol. 93, 1057–1069. https://doi.org/10.1002/jmv.26398 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • CDC. Coronavirus Disease 2019 in Children, https://doi.org/10.15585/mmwr.mm6914e4

  • Götzinger, F. et al. COVID-19 in children and adolescents in europe: a multinational, multicentre cohort study. Lancet Child. Adolesc. Health. 4, 653–661. https://doi.org/10.1016/s2352-4642(20)30177-2 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Hagmann, S. H. F. COVID-19 in children: more than meets the eye. Travel Med. Infect. Dis. 34, 101649. https://doi.org/10.1016/j.tmaid.2020.101649 (2020).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Castagnoli, R. et al. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children and adolescents: a systematic review. JAMA Pediatr. 174, 882–889. https://doi.org/10.1001/jamapediatrics.2020.1467 (2020).

    Article 
    PubMed 

    Google Scholar
     

  • Centre-for-Health-Informatics. Alberta’s Pandemic Response: COVID-19 in Wastewater, (2024). https://covid-tracker.chi-csm.ca/alberta

  • Alberta-Government. Resource Guide for COVID-19 Cases in School (k-12) Settings, (2021). https://open.alberta.ca/publications/resource-guide-covid-19-cases-schools-settings-before-during-after-covid-19-alert-outbreak

  • CBE. Health & Wellness in School, (2024). https://cbe.ab.ca/about-us/school-culture-and-environment/health-and-wellness-in-school/Pages/coronavirus.aspx

  • Alberta-Government. Parents’ Guide 2020–21 School Year, (2020). https://open.alberta.ca/publications/covid-19-information-parents-guide-2020-21-school-year

  • Hassard, F. et al. Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: near-source monitoring of schools in England over an academic year. PLOS One. 18, e0286259. https://doi.org/10.1371/journal.pone.0286259 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lopez Marin, M. A. et al. Monitoring COVID-19 spread in selected prague’s schools based on the presence of SARS-CoV-2 RNA in wastewater. Sci. Total Environ. 871, 161935. https://doi.org/10.1016/j.scitotenv.2023.161935 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wolken, M. et al. Wastewater surveillance of SARS-CoV-2 and influenza in preK-12 schools shows school, community, and citywide infections. Water Res. 231, 119648. https://doi.org/10.1016/j.watres.2023.119648 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Crowe, J. et al. Assessment of a program for SARS-CoV-2 screening and environmental monitoring in an urban public school district. JAMA Netw. Open. 4, e2126447–e2126447. https://doi.org/10.1001/jamanetworkopen.2021.26447 (2021).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Fielding-Miller, R. et al. Safer at school early alert: an observational study of wastewater and surface monitoring to detect COVID-19 in elementary schools. Lancet Reg. Health Am. 19, 100449. https://doi.org/10.1016/j.lana.2023.100449 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Nakgul, L. et al. Saliva and wastewater surveillance for SARS-CoV-2 during school reopening amid COVID-19 pandemic in Thailand. Public. Health Pract. 5, 100378. https://doi.org/10.1016/j.puhip.2023.100378 (2023).

    Article 

    Google Scholar
     

  • Kim, S. & Boehm, A. B. Wastewater monitoring of SARS-CoV-2 RNA at K-12 schools: comparison to pooled clinical testing data. PeerJ 11, e15079. https://doi.org/10.7717/peerj.15079 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kappus-Kron, H. et al. Precision public health in schools enabled by wastewater surveillance: a case study of COVID-19 in an upstate New York middle-high school campus during the 2021–2022 academic year. PLOS Global Public. Health. 4, e0001803. https://doi.org/10.1371/journal.pgph.0001803 (2024).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sellers, S. C. et al. Building-level wastewater surveillance of SARS-CoV-2 is associated with transmission and variant trends in a university setting. Environ. Res. 215, 114277. https://doi.org/10.1016/j.envres.2022.114277 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gibas, C. et al. Implementing building-level SARS-CoV-2 wastewater surveillance on a university campus. Sci. Total Environ. 782, 146749. https://doi.org/10.1016/j.scitotenv.2021.146749 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Harris-Lovett, S. et al. Wastewater surveillance for SARS-CoV-2 on college campuses: initial efforts, lessons learned, and research needs. Int. J. Environ. Res. Public Health. 18, 4455 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kotay, S. M. et al. Building-Level wastewater surveillance for SARS-CoV-2 in occupied university dormitories as an outbreak forecasting tool: one year case study. ACS ES&T Water. 2, 2094–2104. https://doi.org/10.1021/acsestwater.2c00057 (2022).

    Article 
    CAS 

    Google Scholar
     

  • Rondeau, N. C. et al. Building-Level detection threshold of SARS-CoV-2 in wastewater. Microbiol. Spectr. 11, e02929–e02922. https://doi.org/10.1128/spectrum.02929-22 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zambrana, W. et al. SARS-CoV-2 RNA and N antigen quantification via wastewater at the campus level, Building cluster level, and Individual-Building level. ACS ES&T Water. https://doi.org/10.1021/acsestwater.2c00050 (2022).

    Article 

    Google Scholar
     

  • Lee, J. et al. Campus node-based wastewater surveillance enables COVID-19 case localization and confirms lower SARS-CoV-2 burden relative to the surrounding community. Water Res. 244, 120469. https://doi.org/10.1016/j.watres.2023.120469 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Hassard, F., Singh, S., Coulon, F. & Yang, Z. Can wastewater monitoring protect public health in schools? Lancet Reg. Health – Americas. 20 https://doi.org/10.1016/j.lana.2023.100475 (2023).

  • Jones, D. L. et al. Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19. Sci. Total Environ. 749, 141364. https://doi.org/10.1016/j.scitotenv.2020.141364 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lundblad, B. & Hellström, A. L. Perceptions of school toilets as a cause for irregular toilet habits among schoolchildren aged 6 to 16 years. J. Sch. Health. 75, 125–128. https://doi.org/10.1111/j.1746-1561.2005.tb06656.x (2005).

    Article 
    PubMed 

    Google Scholar
     

  • Wolfe, M. K. et al. Wastewater-Based Detection of an Influenza Outbreak (Cold Spring Harbor Laboratory, 2022).

  • Lin, X. et al. Continuous detection of Norovirus and astrovirus in wastewater in a coastal City of China in 2014–2016. Lett. Appl. Microbiol. 73, 418–425. https://doi.org/10.1111/lam.13530 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Benschop, K. S. M. et al. Polio and measles down the drain: environmental enterovirus surveillance in the netherlands, 2005 to 2015. Appl. Environ. Microbiol. 83 https://doi.org/10.1128/aem.00558-17 (2017).

  • Hughes, B. et al. Respiratory syncytial virus (RSV) RNA in wastewater settled solids reflects RSV clinical positivity rates. Environ. Sci. Technol. Lett. 9, 173–178. https://doi.org/10.1021/acs.estlett.1c00963 (2022).

    Article 
    CAS 

    Google Scholar
     

  • Huisman, J. S. et al. Wastewater-based estimation of the effective reproductive number of SARS-CoV-2. Environ. Health Perspect. 130, 057011. https://doi.org/10.1289/EHP10050 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Folkes, M. et al. Campus source to sink wastewater surveillance of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Curr. Res. Microb. Sci. 6, 100240. https://doi.org/10.1016/j.crmicr.2024.100240 (2024).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Joung, M. J. et al. Coupling wastewater-based epidemiological surveillance and modelling of SARS-COV-2/COVID-19: practical applications at the public health agency of Canada. Can. Commun. Dis. Rep. 49, 166–174. https://doi.org/10.14745/ccdr.v49i05a01 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Smith, T. et al. Combining community wastewater genomic surveillance with state clinical surveillance: a framework for SARS-CoV-2 public health practice. Food Environ. Virol. 14, 410–416. https://doi.org/10.1007/s12560-022-09531-2 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zimmermann, P. & Curtis, N. Why is COVID-19 less severe in children? A review of the proposed mechanisms underlying the age-related difference in severity of SARS-CoV-2 infections. Arch. Dis. Child. 106, 429–439. https://doi.org/10.1136/archdischild-2020-320338 (2021).

    Article 
    PubMed 

    Google Scholar
     

  • Hassard, F., Lundy, L., Singer, A. C., Grimsley, J. & Di Cesare, M. Innovation in wastewater near-source tracking for rapid identification of COVID-19 in schools. Lancet Microbe. 2, e4–e5. https://doi.org/10.1016/s2666-5247(20)30193-2 (2021).

    Article 
    PubMed 

    Google Scholar
     

  • Alberta COVID-19 Alberta Statistics, (2021). https://www.alberta.ca/stats/covid-19-alberta-statistics.htm

  • Alberta Alberta Schools to Resume In-person Classes on Jan. 11, as Other COVID-19 Restrictions Extended, (2021).

  • Whitney, O. N. et al. Sewage, salt, silica, and SARS-CoV-2 (4S): an economical kit-free method for direct capture of SARS-CoV-2 RNA from wastewater. Environ. Sci. Technol. 55, 4880–4888. https://doi.org/10.1021/acs.est.0c08129 (2021). Sewage.

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Espinosa, M. F. et al. Reduction and liquid-solid partitioning of SARS-CoV-2 and adenovirus throughout the different stages of a pilot-scale wastewater treatment plant. Water Res. 212, 118069. https://doi.org/10.1016/j.watres.2022.118069 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bastardo-Méndez, M. et al. Detection of SARS-CoV-2 in wastewater as an earlier predictor of COVID-19 epidemic peaks in Venezuela. Sci. Rep. 14, 27294. https://doi.org/10.1038/s41598-024-78982-3 (2024).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Yeager, R. et al. Wastewater sample site selection to estimate geographically resolved community prevalence of COVID-19: a sampling protocol perspective. GeoHealth 5, e2021GH000420. https://doi.org/10.1029/2021GH000420 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bustin, S. A. et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55, 611–622. https://doi.org/10.1373/clinchem.2008.112797 (2009).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Medema, G., Heijnen, L., Elsinga, G., Italiaander, R. & Brouwer, A. Presence of SARS-Coronavirus-2 RNA in sewage and correlation with reported COVID-19 prevalence in the early stage of the epidemic in the Netherlands. Environ. Sci. Technol. Lett. 7, 511–516 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Randazzo, W. et al. SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area. Water Res. 181, 115942. https://doi.org/10.1016/j.watres.2020.115942 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Staley, C., Gordon, K. V., Schoen, M. E. & Harwood, V. J. Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters. Appl. Environ. Microbiol. 78, 7317–7326. https://doi.org/10.1128/aem.01430-12 (2012).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • USEPA. Total, fixed, and volatile solids in water, solids, and biosolids. (2001).

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