Stock Ticker

Host gene expression analysis in the detection of bacterial and viral etiology in children hospitalized with a suspected severe infection

  • Jansen, R. et al. Frequent detection of respiratory viruses without symptoms: toward defining clinically relevant cutoff values. J. Clin. Microbiol. 49, 2631–2636 (2011).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rhedin, S. et al. Clinical utility of PCR for common viruses in acute respiratory illness. Pediatrics 133, e538–e545 (2014).

    Article 
    PubMed 

    Google Scholar
     

  • Byington, C. L. et al. Community surveillance of respiratory viruses among families in the Utah better identification of germs-longitudinal viral epidemiology (BIG-LoVE) study. Clin. Infect. Dis. 61, 1217–1224 (2015).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Relman, D. A. New technologies, human-microbe interactions, and the search for previously unrecognized pathogens. J. Infect. Dis. 186, 254–258 (2002).

    Article 

    Google Scholar
     

  • Iroh Tam, P., Bernstein, E., Ma, X. & Ferrieri, P. Blood culture in evaluation of pediatric community-acquired pneumonia: a systematic review and meta-analysis. Hosp. Pediatr. 5, 324–336 (2015).

    Article 
    PubMed 

    Google Scholar
     

  • Van Den Bruel, A. et al. Diagnostic value of laboratory tests in identifying serious infections in febrile children: systematic review. BMJ 342, d3082 (2011).

    Article 
    PubMed 

    Google Scholar
     

  • Huang, D. T. et al. Procalcitonin-guided use of antibiotics for lower respiratory tract infection. N. Engl. J. Med. 379, 236–249 (2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Engelmann, I. et al. Diagnosis of viral infections using myxovirus resistance protein A (MxA). Pediatrics 135, e985–e993 (2015).

    Article 
    PubMed 

    Google Scholar
     

  • Rhedin, S. et al. Myxovirus resistance protein A for discriminating between viral and bacterial lower respiratory tract infections in children: the TREND study. Clin. Microbiol. Infect. 28, 1251–1257 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Piri, R. et al. Myxovirus resistance protein A as a marker of viral cause of illness in children hospitalized with an acute infection. Microbiol. Spectr. 10, e0203121 (2022).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Piri, R. et al. Evaluation of a novel point-of-care blood myxovirus resistance protein A measurement for the detection of viral infection at the pediatric emergency department. J. Infect. Dis. https://doi.org/10.1093/infdis/jiae367 (2024).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Herberg, J. A. et al. Diagnostic test accuracy of a 2-transcript host RNA signature for discriminating bacterial vs viral infection in febrile children. JAMA 316, 835–845 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Mahajan, P. et al. Association of RNA biosignatures with bacterial infections in febrile infants aged 60 days or younger. JAMA 316, 846–857 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kaforou, M., Herberg, J. A., Wright, V. J., Coin, L. J. M. & Levin, M. Diagnosis of bacterial infection using a 2-transcript host RNA signature in febrile infants 60 days or younger. JAMA 317, 1577–1578 (2017).

    Article 
    PubMed 

    Google Scholar
     

  • Ramilo, O. et al. Gene expression patterns in blood leukocytes discriminate patients with acute infections. Blood 109, 2066–2077 (2007).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Zaas, A. K. et al. Gene expression signatures diagnose influenza and other symptomatic respiratory viral infections in humans. Cell Host Microbe 6, 207–217 (2009).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Hu, X., Yu, J., Crosby, S. D. & Storch, G. A. Gene expression profiles in febrile children with defined viral and bacterial infection. Proc. Natl. Acad. Sci. USA 110, 12792–12797 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sweeney, T. E., Wong, H. R. & Khatri, P. Robust classification of bacterial and viral infections via integrated host gene expression diagnostics. Sci. Transl. Med. 8, 346ra91 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sampson, D. L. et al. A four-biomarker blood signature discriminates systemic inflammation due to viral infection versus other etiologies. Sci. Rep. 7, 1–17 (2017).

    Article 
    CAS 

    Google Scholar
     

  • Lydon, E. C. et al. Validation of a host response test to distinguish bacterial and viral respiratory infection. EBioMedicine 48, 453–461 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Tsalik, E. L. et al. Discriminating bacterial and viral infection using a rapid host gene expression test. Crit. Care Med. 49, 1651–1663 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Habgood-Coote, D. et al. Diagnosis of childhood febrile illness using a multi-class blood RNA molecular signature. Med 4, 635–654 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Rao, A. M. et al. A robust host-response-based signature distinguishes bacterial and viral infections across diverse global populations. Cell Rep. Med. 3, 100842 (2022).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ravichandran, S. et al. VB10, a new blood biomarker for differential diagnosis and recovery monitoring of acute viral and bacterial infections. EBioMedicine 67, 103352 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Toivonen, L. et al. Blood MxA protein as a marker for respiratory virus infections in young children. J. Clin. Virol. 62, 8–13 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Toivonen, L. et al. Rhinovirus infections in the first 2 years of life. Pediatrics 138, e20161309 (2016).

    Article 
    PubMed 

    Google Scholar
     

  • Katayama, S. et al. Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods. BMC Bioinform. 20, 1–9 (2019).

    Article 
    CAS 

    Google Scholar
     

  • Ezer, S. et al. Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog. STAR Protoc. 2, 100995 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Krjutškov, K. et al. Single-cell transcriptome analysis of endometrial tissue. Hum. Reprod. 31, 844–853 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Katayama, S., Töhönen, V., Linnarsson, S. & Kere, J. SAMstrt: statistical test for differential expression in single-cell transcriptome with spike-in normalization. Bioinformatics 29, 2943–2945 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Erkeller-Yuksel, F. M. et al. Age-related changes in human blood lymphocyte subpopulations. J. Pediatr. 120, 216–222 (1992).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Wong, H. R. et al. Genomic expression profiling across the pediatric systemic inflammatory response syndrome, sepsis, and septic shock spectrum. Crit. Care Med. 37, 1558–1566 (2010).

    Article 

    Google Scholar
     

  • Huang, L., Qiao, L., Zhu, H., Jiang, L. & Yin, L. Genomics of neonatal sepsis: has-miR-150 targeting BCL11B functions in disease progression. Ital. J. Pediatr. 44, 145 (2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ming, T. et al. Integrated analysis of gene co-expression network and prediction model indicates immune-related roles of the identified biomarkers in sepsis and sepsis-induced acute respiratory distress syndrome. Front. Immunol. 13, 897390 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Herberg, J. A. et al. Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes. J. Infect. Dis. 208, 1664–1668 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Batarseh, A. & Papadopoulos, V. Regulation of translocator protein 18kDa (TSPO) expression in health and disease states. Mol. Cell. Endocrinol. 327, 1–12 (2010).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Papadopoulos, V. et al. Translocator protein (18 kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function. Trends Pharmacol. Sci. 27, 402–409 (2006).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Betlazar, C., Middleton, R. J., Banati, R. & Liu, G. J. The translocator protein (TSPO) in mitochondrial bioenergetics and immune processes. Cells 9, 512 (2020).

  • Blevins, L. K., Crawford, R. B., Azzam, D. J., Guilarte, T. R. & Kaminski, N. E. Surface translocator protein 18 kDa (TSPO) localization on immune cells upon stimulation with LPS and in ART-treated HIV+ subjects. J. Leukoc. Biol. 110, 123–140 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Veenman, L., Shandalov, Y. & Gavish, M. VDAC activation by the 18 kDa translocator protein (TSPO), implications for apoptosis. J. Bioenerg. Biomembr. 40, 199–205 (2008).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Banati, R. B. et al. Positron emission tomography and functional characterization of a complete PBR/TSPO knockout. Nat. Commun. 5, 5452 (2014).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rupprecht, R. et al. Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders. Nat. Rev. Drug Discov. 9, 971–988 (2010).

  • Colasanti, A. et al. In vivo assessment of brain white matter inflammation in multiple sclerosis with 18F-PBR111 PET. J. Nucl. Med. 55, 1112–1118 (2014).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Hatori, A. et al. PET imaging of lung inflammation with [18F]FEDAC, a radioligand for translocator protein (18 kDa). PLoS ONE 7, e45065 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Giridharan, V. V. et al. A crosstalk between gut and brain in sepsis-induced cognitive decline. J. Neuroinflammation 19, 114 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Giga, H. et al. Pharmacological and genetic inhibition of translocator protein 18 kDa ameliorated neuroinflammation in murine endotoxemia model. Shock 56, 142–149 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Jin, G. lin et al. Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice. Phytomedicine 107, 154484 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Leaver, K. R. et al. Effects of translocator protein (18 kDa) ligands on microglial activation and neuronal death in the quinolinic-acid-injected rat striatum. ACS Chem. Neurosci. 3, 114–119 (2012).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Barron, A. M. et al. Ligand for translocator protein reverses pathology in a mouse model of Alzheimer’s disease. J. Neurosci. 33, 8891–8897 (2013).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Simon-O’Brien, E., Gauthier, D., Riban, V. & Verleye, M. Etifoxine improves sensorimotor deficits and reduces glial activation, neuronal degeneration, and neuroinflammation in a rat model of traumatic brain injury. J. Neuroinflammation 13, 203 (2016).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Leva, G. et al. The translocator protein ligand XBD173 improves clinical symptoms and neuropathological markers in the SJL/J mouse model of multiple sclerosis. Biochim. Biophys. Acta Mol. Basis Dis. 1863, 3016–3027 (2017).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Ma, B. et al. Translocator protein agonist Ro5-4864 alleviates neuropathic pain and promotes remyelination in the sciatic nerve. Mol. Pain 14, 1744806917748019 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Monga, S., Nagler, R., Amara, R., Weizman, A. & Gavish, M. Inhibitory effects of the two novel TSPO ligands 2-Cl-MGV-1 and MGV-1 on LPS-induced microglial activation. Cells 8, 486 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Dominguini, D. et al. The protective effect of PK-11195 on cognitive impairment in rats survived of polymicrobial sepsis. Mol. Neurobiol. 58, 2724–2733 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Copeland, P. R., Fletcher, J. E., Carlson, B. A., Hatfield, D. L. & Driscoll, D. M. A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs. EMBO J. 19, 306–314 (2000).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rayman, M. P. Selenium and human health. Lancet 379, 1256–1268 (2012).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Avery, J. C. & Hoffmann, P. R. Selenium, selenoproteins, and immunity. Nutrients 10, 1203 (2018).

  • Guillin, O. M., Vindry, C., Ohlmann, T. & Chavatte, L. Selenium, selenoproteins and viral infection. Nutrients 11, 2101 (2019).

  • Fairweather-Tait, S. J. et al. Selenium in human health and disease. Antioxid. Redox Signal. 14, 1337–1383 (2011).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Bae, M. & Kim, H. Mini-review on the roles of vitamin C, vitamin D, and selenium in the immune system against COVID-19. Molecules 25, 5346 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Hoffmann, F. K. W. et al. Dietary selenium modulates activation and differentiation of CD4+ T cells in mice through a mechanism involving cellular free thiols. J. Nutr. 140, 1155–1161 (2010).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kiremidjian-Schumacher, L., Roy, M., Harvey, W., Martin, C. & Stotzky, G. Supplementation with selenium and human immune cell functions. I. Effect on lymphocyte proliferation and interleukin 2 receptor expression. Biol. Trace Elem. Res. 41, 103–104 (1994).

    Article 
    PubMed 

    Google Scholar
     

  • Beck, M. et al. Selenium deficiency increases the pathology of an influenza virus infection. FASEB J. 15, 1481–1483 (2001).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Beck, M. A. et al. Benign human enterovirus becomes virulent in selenium-deficient mice. J. Med. Virol. 43, 166–170 (1994).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Moghaddam, A. et al. Selenium deficiency is associated with mortality risk from COVID-19. Nutrients 12, 1–13 (2020).

    Article 

    Google Scholar
     

  • Khan, M., Dilawar, S., Ali, I. & Rauf, N. The possible role of selenium concentration in hepatitis B and C patients. Saudi J. Gastroenterol. 18, 106–110 (2012).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Stone, C. A., Kawai, K., Kupka, R. & Fawzi, W. W. Role of selenium in HIV infection. Nutr. Rev. 68, 671–681 (2010).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bodkin, N. et al. Systematic comparison of published host gene expression signatures for bacterial/viral discrimination. Genome Med. 14, 1–14 (2022).

    Article 

    Google Scholar
     

  • Ko, E. R. et al. Host-response transcriptional biomarkers accurately discriminate bacterial and viral infections of global relevance. Sci. Rep. 13, 22554 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ko, E. R. et al. Prospective validation of a rapid host gene expression test to discriminate bacterial from viral respiratory infection. JAMA Netw. Open 5, e227299 (2022).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Barral-Arca, R., Pardo-Seco, J., Martinón-Torres, F. & Salas, A. A 2-transcript host cell signature distinguishes viral from bacterial diarrhea and it is influenced by the severity of symptoms. Sci. Rep. 8, 4–10 (2018).

    Article 

    Google Scholar
     

  • Pennisi, I. et al. Discrimination of bacterial and viral infection using host-RNA signatures integrated in a lab-on-chip platform. Biosens. Bioelectron. 216, 114633 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Shah, P. et al. Relationship between molecular pathogen detection and clinical disease in febrile children across Europe: a multicentre, prospective observational study. Lancet Reg. Health Eur. 26, 100682 (2023).

    Article 

    Google Scholar
     

  • Source link

    Get RawNews Daily

    Stay informed with our RawNews daily newsletter email

    Late Influencer Ashlee Jenae’s Family Speaks Out on Mysterious Death

    Trump flags prolonged fuel price pain, through November!, as US escalates Iran blockade

    Confirmed teams and full line ups in Premier League, TV channel, live streams

    Britney Spears in Rehab for Substance Abuse